Development and<i> in vitro </i>characterization nanoemulsion containing the methanol extract of <i>Hypericum linarioides </i>for wound healing: <i>In vitro </i>scratch assay

It was aimed to develop and in vitro characterize the methanol extract of Hypericum linarioides (MHL)-containing nanoemulsion formulation (MHL-NEF) to heal the wound effectively. MHL-NEF was prepared and its droplet size, polydispersity index (PDI), viscosity, pH and zeta potential (ZP) values were determined. The effect of MHL and MHL-NEF on human dermal fibroblast cell viability was examined by MTT assay. Also, their wound healing potential was evaluated using the scratch assay. The droplet size, PDI, ZP, pH and viscosity values for the MHL-NEF were found to be 157.67 +/- 7.61 nm, 0.268 +/- 0.036, (-)40.20 +/- 6.79 mV, 4.27 +/- 0.01, and 1.82 +/- 0.01 cP, respectively. In the cell viability assay, the highest percentage of living cells was obtained at concentration of 0.4 mu g/mL for the MHL and MHL-NEF. In the scratch assay, MHL-NEF showed significantly ameliorated wound closure (%) rates at 24 and 30 h than the groups of control, MHL and blank-NEF (NEF without MHL) (p < 0.001). Immunofluorescence staining was carried out. TGF-beta 1 and FGFR-2 expressions in MHL-NEF-treated fibroblast cells increased significantly at 24 h compared to the groups of control, MHL, and B-NEF (p < 0.001). Our findings show that MHL-NEF may be promising for better wound healing. (c) 2024 SAAB. Published by Elsevier B.V. All rights reserved.

Dergi

SOUTH AFRICAN JOURNAL OF BOTANY

Yıl

2024

Cilt

168

Sayfa

316-327

ISSN

0254-6299

eISSN

1727-9321

DOI

10.1016/j.sajb.2024.03.026

WoS UID

WOS:001222838100001