Article 2024 · 2 atıf

Investigation of the protective effect of chitosan against arsenic-induced nephrotoxicity and oxidative damage in rat kidney tissue

Irak, K., Celik, O. Y., Bolacali, M., Tufan, T., Ozcan, C., Yildirim, S., Bolat, I., Bolat, I.

Özet

Arsenic is an important metalloid that can cause poisoning in humans and domestic animals. Exposure to arsenic causes cell damage, increasing the production of reactive oxygen species. Chitosan is a biopolymer obtained by deacetylation of chitin with antioxidant and metal ion chelating properties. In this study, the protective effect of chitosan on arsenic -induced nephrotoxicity and oxidative damage was investigated. 32 male Wistar-albino rats were divided into 4 groups of 8 rats each as control group (C), chitosan group (CS group), arsenic group (AS group), and arsenic+chitosan group (AS+CS group). The C group was given distilled water by oral gavage, the AS group was given 100 ppm/day Na-arsenite ad libitum with drinking water, the CS group was given 200 mg/kg/day chitosan dissolved in saline by oral gavage, the AS+CS group was given 100 ppm/day Na-arsenite ad libitum with drinking water and 200 mg/kg/day chitosan dissolved in saline by oral gavage for 30 days. At the end of the 30 -day experimental period, 90 mg/kg ketamine was administered intraperitoneally to all rats, and blood samples and kidney tissues were collected. Urea, uric acid, creatinine, P, Mg, K, Ca, Na, Cystatin C (CYS-C), Neutrophil Gelatinase Associated Lipocalin (NGAL) and Kidney Injury Molecule 1 (KIM -1) levels were measured in serum samples. Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT) and Superoxide dismutase (SOD) levels in the supernatant obtained from kidney tissue were analyzed by ELISA method. Compared with AS group, uric acid and creatinine levels of the AS+CS group were significantly decreased (p<0.001), urea, KIM -1, CYS-C, NGAL, and MDA levels were numerically decreased and CAT, GSH, and SOD levels were numerically increased (p>0.05). In conclusion, based on both biochemical and histopathological-immunohistochemical-immunofluorescence findings, it can be concluded that chitosan attenuates kidney injury and protects the kidney.

Anahtar Kelimeler

arsenic chitosan nephrotoxicity oxidative stress rat CYSTATIN C INDUCED APOPTOSIS STRESS EXPOSURE INJURY CELLS GENOTOXICITY DERIVATIVES TOXICITY PROTEIN

WoS Kategorileri

Veterinary Sciences

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam Temiz Su ve Sıhhi Koşullar

Künye

Dergi: POLISH JOURNAL OF VETERINARY SCIENCES
Yıl: 2024
Cilt: 27
Sayı: 1
Sayfa: 95-105
ISSN: 1505-1773
eISSN: 2300-2557
DOI: 10.24425/pjvs.2024.149339
WoS UID: WOS:001189097600007

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