Article 2024 · 14 atıf

Zingerone attenuates sciatic nerve damage caused by sodium arsenite by inhibiting NF-κB, Caspase-3, and ATF-6/CHOP pathways and activating the Akt-2/FOXO1 pathway

Yilmaz, Selcuk, Gur, Cihan, Kucukler, Sefa, Akaras, Nurhan, Kandemir, Fatih Mehmet

Özet

Objective(s): In the present study, the potential protective effects of zingerone (ZNG) against sciatic nerve damage caused by sodium arsenite (SA), a common environmental pollutant, were evaluated by various biochemical, molecular, and histological methods.<br /> Materials and Methods: In the study, SA and ZNG were given to 35 male Sprague Dawley rats for 14 days. At the end of the period, the sciatic nerve tissues were taken and the markers involved in oxidative stress, endoplasmic reticulum stress, inflammation, and apoptosis were analyzed.<br /> Results: The data obtained showed that SA decreased glutathione (GSH) levels and increased malondialdehyde (MDA) levels in the sciatic nerve tissue. However, it was determined that these markers approached the control group levels due to the anti-oxidant properties of ZNG. While SA triggered endoplasmic reticulum stress and apoptosis pathways, ZNG suppressed them. Moreover, SA up-regulated inflammatory markers such as nuclear factor kappa-B (NF-kappa B), tumor necrosis factor-alpha (TNF-alpha), interleukin-1-beta (IL-1 beta), and neuronal nitric oxide synthases (nNOS) in the sciatic nerves and caused neuro-inflammation and inhibited cell survival by suppressing serine/threonine-protein kinase 2 (Akt2) and forkhead box protein O1 (FOXO1) genes. It has also been shown histopathologically that SA causes degeneration in the sciatic nerves. In contrast, ZNG suppressed neuro-inflammation, activated Akt2/FOXO1 signaling, and repaired histological irregularities.<br /> Conclusion: In general, SA caused oxidative stress, inflammation, ER stress, and apoptosis in the sciatic nerves of rats, causing damage to the tissues, however, ZNG suppressed these pathways and protected the sciatic nerves from the destructive effect of SA.

Anahtar Kelimeler

Apoptosis Endoplasmic reticulum- stress Inflammation Sciatic nerve Sodium arsenite Zingerone OXIDATIVE STRESS INDUCED APOPTOSIS CELL-DEATH EXPOSURE GROWTH INJURY

WoS Kategorileri

Medicine, Research & Experimental Pharmacology & Pharmacy

Sürdürülebilir Kalkınma Amaçları

Temiz Su ve Sıhhi Koşullar Sağlık ve Kaliteli Yaşam

Künye

Dergi: IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
Yıl: 2024
Cilt: 27
Sayı: 4
Sayfa: 485-491
ISSN: 2008-3866
eISSN: 2008-3874
DOI: 10.22038/IJBMS.2023.74088.16094
WoS UID: WOS:001157865300008

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