Article 2024 · 5 atıf

Plant growth-promoting bacteria isolated from earthworms enhance spinach growth and its phytoremediation potential in metal-contaminated soils

Houida, Sofia, Yakkou, Lamia, Kaya, Leyla Okyay, Bilen, Serdar, Raouane, Mohamed, El Harti, Abdellatif, Amghar, Souad

Özet

The aim of this study was to evaluate the impact of metal-tolerant plant growth-promoting bacteria (PGPB) isolated from the chloragogenous tissue of Aporrectodea molleri, which represents a unique habitat. Our objectives were to investigate their effects on the growth of Spinacia oleracea under heavy metal stress and assess their potential for enhancing phytoremediation capabilities. The experiment was conducted in an alkaline soil contaminated with 7 mg kg(-1) of cadmium, 100 mg kg(-1) of nickel, 150 mg kg(-1) of copper, 300 mg kg(-1) of Zinc, and mg kg(-1) of 600 Manganese. The results showed that heavy metal stress considerably diminished root (42.8%) and shoot length (60.1%), biomass (80%), chlorophyll content (41%), soil alkaline (45%), and acid (51%) phosphatases (42%) and urease (42%). However, soil inoculation with bacterial isolates remarkably improved plant growth. Soil bioaugmentation increased spinach growth (up to 74.5% for root length, up to 106.3% for shoot length, and up to 5.5 folds for fresh biomass) while significantly increasing soil enzyme activity and NPK content. Multivariate data analysis indicated that soil inoculation with Bacillus circulans TC7 promoted plant growth while limiting metal bioaccumulation, whereas Pseudomonas sp. TC33 and Bacillus subtilis TC34 increased metal bioaccumulation in spinach tissues while minimizing their toxicity. Our study confirms that earthworms are a reservoir of multi-beneficial bacteria that can effectively improve phytoremediation efficiency and mitigate the toxic effects of heavy metals on plant growth. Further studies are needed to investigate the long-term effects and feasibility of using these isolates as a consortium in field applications.

Anahtar Kelimeler

Phytoremediation Earthworm-associated bacteria Heavy-metal tolerant bacteria Plant Growth-Promoting bacteria Heavy metal stress Spinach SOLANUM-NIGRUM L. HEAVY-METALS ACCUMULATION CADMIUM CD RHIZOSPHERE TISSUE

WoS Kategorileri

Biotechnology & Applied Microbiology Microbiology

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam

Künye

Dergi: INTERNATIONAL MICROBIOLOGY
Yıl: 2024
Cilt: 27
Sayı: 2
Sayfa: 545-558
ISSN: 1139-6709
eISSN: 1618-1905
DOI: 10.1007/s10123-023-00402-9
WoS UID: WOS:001039324500002

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