Effects of Amifostine Application on Mucin 1 Secretion in Prostate Tissue of Radiotherapy-treated Rats

OBJECTIVE This experimental study was aimed to assess the positive or negative effects of the radioprotective agent amifostine (AMF) (WR-2721) on mucin 1 (MUC1) through immunohistochemical methods in preventing the damage that may occur in the prostate tissue after radiotherapy (RT).METHODS In the experimental period, 32 Sprague-Dawley male rats were divided into 4 groups: Untreated control group, AMF group; 200 mg AMF was intraperitoneally administered to the rats in the second group for 3 days. The rats in the third group (RT-saline) were experimentally administered a total of 6 Gy including 2 Gy of RT in 3 fractions. The rats in the fourth group (RT - AMF) received totally 6 Gy RT and 200 mg/kg AMF for 3 days. The rats were sacrificed for histological assessments 4 weeks after RT. Triple staining was performed to the tissues for histopathological examinations and immunohistochemical localization of MUC1 in prostate tissue was determined using streptavidin-biotin peroxidase method.RESULTS Histopathologically, it was observed that the parenchymal and stromal cells of the prostate tissues in all groups had a structurally normal appearance. Immunohistochemically, the MUC1 immunoreactivity was weak in the parenchymal and stromal cells of the control group, and strong MUC1 secretion was observed in the AMF group, AMF RT group, and RT group.CONCLUSION When considering the effects of AMF application on MUC1 secretion in the prostate tissue of rats treated with RT, it is thought that the protective effect of AMF may vary in tissues, depending on the dose applied and the number of fractions. Keywords: Amifostine; mucin 1; prostate; radiotherapy; rat. Copyright & COPY; 2023, Turkish Society for Radiation Oncology

Dergi

TURK ONKOLOJI DERGISI-TURKISH JOURNAL OF ONCOLOGY

Yıl

2023

ISSN

1300-7467

DOI

10.5505/tjo.2023.3974

WoS UID

WOS:001011935100001