Article 2023 · 2 atıf

Evaluation of wool protein hydrolysate as peptone for production of microbial enzymes

Omeroglu, Mehmet Akif, Albayrak, Seyda, Arslan, Nazli Pinar, Ozkan, Hakan, Adiguzel, Ahmet, Taskin, Mesut

Özet

Peptones are one of the most expensive components of microbial culture media. The present study was conducted to test the usability of low-cost sheep wool peptone (SWP) as an organic nitrogen source in the production of six industrially important enzymes (lipase, amylase, tannase, pectinase, cellulase and invertase). SWP was prepared by alkaline hydrolysis and acid neutralization. Bacillus licheniformis and Aspergillus niger were selected as test microorganisms for enzyme production. To evaluate the efficacy of SWP in enzyme production, it was compared with commercial tryptone peptone (TP) in the shaking flask cultures of the test microorganisms. The optimum concentration of both SWP and TP was determined to be 8 g/L for the production of B. licheniformis-derived enzymes, but 6 g/L for the production of A. niger-derived enzymes. It was determined that SWP was superior to TP in the production of four enzymes (lipase, amylase, tannase and pectinase) of both B. licheniformis and A. niger. This is the first study about the usage of sheep wool protein hydrolysate (SWP) as an organic nitrogen source or a peptone in fermentative production of microbial enzymes.

Anahtar Kelimeler

Bacillus licheniformis Aspergillus niger Industrial enzymes Keratinous proteins Sheep wool protein hydrolysate SOLID-STATE FERMENTATION WASTE CHICKEN FEATHERS CITRIC-ACID PRODUCTION SUBMERGED FERMENTATION PARTIAL-PURIFICATION CULTURE-CONDITIONS LIPASE PRODUCTION INVERTASE CELLULASE TANNASE

WoS Kategorileri

Biotechnology & Applied Microbiology

Künye

Dergi: 3 BIOTECH
Yıl: 2023
Cilt: 13
Sayı: 1
ISSN: 2190-572X
eISSN: 2190-5738
DOI: 10.1007/s13205-022-03456-0
WoS UID: WOS:000906704900002

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