Article 2023 · 12 atıf

Suberosin Alleviates Sepsis-Induced Lung Injury in A Rat Model of Cecal Ligation and Puncture

Uzuncakmak, Sevgi Karabulut, Halici, Zekai, Karakaya, Songul, Kutlu, Zerrin, Saglam, Yavuz Selim, Bolat, Ismail, Aydin, Pelin, Kilic, Ceyda Sibel

Özet

Background/aims Sepsis is one of the major problems encountered in intensive care units, causing organ damage and increasing mortality. Suberosin (SBR) is a type of coumarin with antioxidant and anti-inflammatory activities. The goal of this study is to explore the protective effects of SBR on the lungs in a rat model of sepsis. Methods Male Wistar rats were utilized in this study. A cecal ligation and puncture (CLP) model was applied to induce sepsis. Rats were separated into six groups with nine animals in each group, including healthy control, SBR, CLP, and CLP + SBR (5, 10, and 20 mg/kg) groups. Superoxide dismutase (SOD), glutathione (GSH) enzyme activities, and malondialdehyde (MDA) level were measured via enzyme-linked immunosorbent assay (ELISA). The messenger RNA (mRNA) expressions of tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) were evaluated by real-time polymerase chain reaction (RT-PCR). Histopathological changes in the lungs were investigated with hematoxylin and eosin (H&E). Results MDA levels and GSH and SOD enzyme activities were negatively affected in the CLP group, but SBR treatment ameliorated these oxidative stress parameters in the SBR1-3 groups (p< 0.05). The mRNA expressions of TNF-alpha and IL-1 beta were increased in the CLP group, and SBR treatment decreased those expression levels in a dose-dependent manner (p < 0.05). Organ damage and necrosis were seen in the CLP group and were alleviated in the SBR3 group. Immunohistochemical (IHC) analysis of lung tissues demonstrated decreased TNF-alpha and IL-1 beta immunopositivity in the SBR1-3 groups (p< 0.05). Conclusions SBR ameliorated sepsis-related lung injury in a dose-dependent manner. This compound has significant potential as a future agent in the treatment of sepsis.

Anahtar Kelimeler

oxidative stress rat sepsis suberosin lung injury FERULAGO COUMARINS ANTIOXIDANT EXPRESSION THERAPY BOISS. ROOTS CELLS

WoS Kategorileri

Surgery

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam

Künye

Dergi: JOURNAL OF INVESTIGATIVE SURGERY
Yıl: 2023
Cilt: 36
Sayı: 1
Sayfa: 1-9
ISSN: 0894-1939
eISSN: 1521-0553
DOI: 10.1080/08941939.2022.2136802
WoS UID: WOS:000879947700001

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