Article 2022 · 16 atıf

Infrared spectrochemical findings on intermittent fasting-associated gross molecular modifications in rat myocardium

Ardahanli, Isa, Ibrahim Ozkan, Halil, Ozel, Faik, Gurbanov, Rafig, Teker, Hikmet Taner, Ceylani, Taha

Özet

Cardiovascular diseases are among the primary life-threatening conditions affecting human society. Intermittent fasting is shown to be functional in the prevention of cardiovascular diseases, however, the information on fasting-associated modifications in myocardial biomolecules is limited. This study aimed to determine the impact of 18-h intermittent fasting administered for five weeks on 12 months-old rats using supervised linear discriminant analysis and support vector machine algorithms constructed on spectrochemical data obtained from myocardial tissues. These algorithms revealed gross biomolecular modifications, while quantitative analyses demonstrated higher amounts of saturated lipids (19%), triglycerides (11%), and lipids (56%), in addition to enhancement in membrane dynamics (18%). The concentrations of nucleic acids and glucose are increased by 52%, while the glycogen content is diminished by 61%. The protein carbonylation/oxidation is reduced by 38%, whereas a 35% increase in protein content was measured. Phosphorylated proteins have been calculated to be at higher concentrations in the 13-62% range. The study findings demonstrated significant molecular changes in the myocardium of rats subjected to intermittent fasting.

Anahtar Kelimeler

Cardiovascular diseases Infrared spectroscopy Intermittent fasting Linear Discriminant Analysis Support Vector Machine OXIDATIVE DAMAGE METABOLISM RESTRICTION MECHANISMS AUTOPHAGY MEMBRANE FLUIDITY RISK MICE

WoS Kategorileri

Biochemistry & Molecular Biology Biophysics Chemistry, Physical

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam

Künye

Dergi: BIOPHYSICAL CHEMISTRY
Yıl: 2022
Cilt: 289
ISSN: 0301-4622
eISSN: 1873-4200
DOI: 10.1016/j.bpc.2022.106873
WoS UID: WOS:000863266200003

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