Article 2022 · 14 atıf

Morin mitigates ifosfamide induced nephrotoxicity by regulation of NF-kappaB/p53 and Bcl-2 expression

Comakli, Selim, Kandemir, Fatih Mehmet, Kucukler, Sefa, Ozdemir, Selcuk

Özet

Ifosfamide (IFO) is used for treating childhood solid tumors, but its use is limited by its adverse effects on kidneys. Morin may be used to prevent nephrotoxic and other side effects. We investigated the underlying mechanisms of the protective effects of morin on IFO induced nephrotoxicity. We used 35 male rats divided into five groups of seven: control group, morin group, IFO group, 100 mg/kg morin + IFO group and 200 mg/kg morin + IFO group. We measured kidney tissue oxidant, antioxidant and inflammatory parameters using ELISA, and apoptosis was evaluated using immunohistochemistry and real time PCR. Serum urea, creatinine and kidney injury molecule-1 (KIM-1) levels were increased by IFO treatment; elevated levels were decreased significantly by treatment with both 100 and 200 mg/kg morin. Morin treatment also decreased oxidative stress and lipid oxidation in IFO treated rats. The ameliorative effect of morin on inflammatory response was due to reduced levels of NF-kappa B and TNF-alpha. Morin also reduced NF-kappa B/p53 levels by increasing Bcl-2 expression in IFO treated kidneys. Morin may prevent IFO induced nephrotoxicity via the NF-kappa B/p53 and Bcl-2 signaling pathways.

Anahtar Kelimeler

Bcl-2 C-fos chloroacetaldehyde ifosfamide kidney KIM-1 morin nephrotoxicity NF-kappa B/p53 rats NF-KAPPA-B INDUCED FANCONI-SYNDROME OXIDATIVE STRESS C-FOS THERAPEUTIC-EFFICACY ANTIOXIDANT ACTIVITY GENE-EXPRESSION APOPTOSIS INFLAMMATION CISPLATIN

WoS Kategorileri

Biotechnology & Applied Microbiology Cell Biology

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam

Künye

Dergi: BIOTECHNIC & HISTOCHEMISTRY
Yıl: 2022
Cilt: 97
Sayı: 6
Sayfa: 423-432
ISSN: 1052-0295
eISSN: 1473-7760
DOI: 10.1080/10520295.2021.2021449
WoS UID: WOS:000744454800001

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