Article 2022 · 10 atıf

Extracellular production of azurin from <i>Pseudomonas aeruginosa</i> in the presence of Triton X-100 or Tween 80

Unver, Yagmur, Yildiz, Seyda, Acar, Melek

Özet

Azurin which is a bacterial secondary metabolite has attracted much attention as potential anticancer agent in recent years. This copper-containing periplasmic redox protein supresses the tumor growth selectively. High-level secretion of proteins into the culture medium offers a significant advantage over periplasmic or cytoplasmic expression. The aim of this study was to investigate the effect of nonionic surfactants on the expression of the Pseudomonas aeruginosa azurin. Different concentrations of Triton X-100 and Tween 80 were used as supplements in growth media and extracellular azurin production was stimulated by both surfactants. According to western blot analysis results, in the presence of Triton X-100, maximum azurin expression level was achieved with 96 h of incubation at 1% concentration, and 48 h at 2% concentration. On the other hand, maximum azurin expression level was achieved in the presence of 1% Tween 80 at 72 h incubation. This study suggested for the first time a high level of azurin secretion from P. aeruginosa in the presence of Triton X-100 or Tween 80, which would be advantageous for the purification procedure.

Anahtar Kelimeler

Pseudomonas aeruginosa Azurin Extracellular secretion Triton X-100 Tween 80 GRAM-NEGATIVE BACTERIA LIPASE PRODUCTION PROTEIN PERMEABILIZATION SECRETION OPTIMIZATION RELEASE ALPHA CELLS

WoS Kategorileri

Biotechnology & Applied Microbiology Engineering, Chemical

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam

Künye

Dergi: BIOPROCESS AND BIOSYSTEMS ENGINEERING
Yıl: 2022
Cilt: 45
Sayı: 3
Sayfa: 553-561
ISSN: 1615-7591
eISSN: 1615-7605
DOI: 10.1007/s00449-021-02678-5
WoS UID: WOS:000743409300001

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