Article 2021 · 19 atıf

A new approach for affinity-based purification of horseradish peroxidase

Almaz, Zuleyha, Oztekin, Aykut, Abul, Nurgul, Gerni, Serpil, Erel, Deniz, Kocak, Seyma Mehtap, Sengul, Mehmet Emin, Ozdemir, Hasan

Özet

We have developed efficient procedure for isolation of horseradish peroxidase (HRP) using aminobenzohydrazide-based affinity chromatography. Sepharose 4B-bounded aminobenzohydrazides are suitable for long-term use and large-scale purification. In this study, 26 aminobenzohydrazide derivatives were synthesized, characterized and defined as new HRP inhibitors. In addition, detailed inhibition effects of these molecules on HRP enzyme were investigated. Affinity matrix was formed by bonding aminobenzohydrazides, which exhibited inhibitory activity to sepharose-4B-l-tyrosine. HRP was isolated from crude homogenate in single step and purification factors were recorded as 1,151-fold (recovery of 8.5%) with 4-amino 3-bromo benzohydrazide and as 166.16-fold (recovery of 16.67 %) with 3-amino 4-chloro benzohydrazide.

Anahtar Kelimeler

affinity purification benzohydrazide horseradish peroxidase GLUTATHIONE-S-TRANSFERASE TURNIP PEROXIDASE LACTOPEROXIDASE ENZYMES BUTYRYLCHOLINESTERASE ACETYLCHOLINESTERASE MEMBRANE PROTEINS L.

WoS Kategorileri

Biochemistry & Molecular Biology Biotechnology & Applied Microbiology

Sürdürülebilir Kalkınma Amaçları

Temiz Su ve Sıhhi Koşullar

Künye

Dergi: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Yıl: 2021
Cilt: 68
Sayı: 1
Sayfa: 102-113
ISSN: 0885-4513
eISSN: 1470-8744
DOI: 10.1002/bab.1899
WoS UID: WOS:000525839900001

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